Block diagram of hplc instrument. The mobile phase is pumped through the column by a pump. The heart of a hplc system is the column. The effluent from the column enters the mass spectrometers ion source using an interface the removes most of the mobile phase an essential need because of the incompatibility between the liquid mobile phase and the mass spectrometers high vacuum environment. Figure 1250 shows a block diagram of a typical hplcms instrument.
Block diagram of hplc experimental setup. A high pressure pump solvent delivery system or solvent manager is used to generate and meter a specified flow rate of mobile phase typically milliliters per minute. Block diagram and explanation emily a basic block diagram of an hplc is shown in figure 1. N n n n ch3 cooh ch3 ococh3 oh nhcoch3 aspirin acetaminophen caffeine o o ch3 n n n n ch3 ch3 caffeine o o ch3.
The heart of the system is the column where separation occurs. Myosmine were prepared and quantified using high performance liquid chromatography hplc. Arrangement of these components is shown in a block diagram in figure 1 and this arrangement is common to virtually all gas chromatographs regardless of age model or manufacturer. It is not only instrumental tlc but entire concept that include widely.
You can edit this block diagram using creately diagramming tool and include in your reportpresentationwebsite. Block diagram of an hplc your desired solvent mixture travels through capillary tubes from the solvent reservoir to the pump where it is becomes highly pressurized. In the animation below the injector injects a mixture of blue and red molecules into the mobile phase. Various elution schemes were tested and adjusted for optimal analyte resolution and a final elution scheme was developed to characterize e cigarette alkaloids.
Instrumentation of hplc as shown in the schematic diagram in figure above hplc instrumentation includes a pump injector column detector and integrator or acquisition and display system. In the discussion below the general function of each component is provided with comments on the status of the technology. Arshad hala seth g. The column contains the particles that contains the stationary phase.
The components of a basic high performance liquid chromatography hplc system are shown in the simple diagram in figure e. Now a day hptlc is more useful than tlc and hplc. A reservoir holds the solvent called the mobile phase because it moves. A block diagram showing hplc block.
Because hptlc is independent of sample application chromatogram development detection etc. The liquid chromatograph.